Allied Sectors and Occupations

Bamboo made house with thatched roof and mud-plastered wall is preferable for grainage.
Disinfect the grainage rooms and appliances prior to commencement of operation.
Wash the rooms and appliances with 5% bleaching powder solution before one day.
Dry the appliances in sun shine for 3-4 hours before one day.
Disinfect the rooms and appliances with a mixture of 2% formalin, 0.5-1% lime and 0.5% detergent solutions @ 1L/ sq.m after one day.

Immediately after the receipt of seed cocoons, spread them on trays in a single layer to facilitate good aeration.
Reject the seed cocoons which are melted, uzi infested, flimsy and not conforming to the characteristics of parent races.
Preserve only the healthy seed cocoons in trays for further processing.
Provide cross ventilation in the preservation room.
Maintain temperature at 25 ± 1˚C, relative humidity of 75 ± 5%, 16 hour light and 8 hour dark conditions in the cocoon preservation room.
Maintain complete darkness one day prior to emergence to avoid irregular emergence of moths.

This helps in determining the disease freeness of a batch and helps in minimizing the loss to grainage.
For early emergence of moths, 50-60 seed cocoons are taken from individual lots and placed into an artificial eclosion box.
Maintain the temperature at 32-33 0C with the help of thermostat. This accelerates the development of pupae and moth emerges early.
Examine the early emerged female moths of respective lots are under microscope to know the disease freeness of lots.

Keep the cocoon preservation room dark before the expected day of emergence of moths.
In case of variation in development of male or female pupae, the development of male pupae can be arrested by preserving them at 5-7 0C and 75 + 5% relative humidity for 3-4 days.
Only healthy and active moths are taken for pairing.
After 1-2 hours of emergence, the male and female moths of respective combination are allowed for 3.5 to 4 hours of pairing.
At the time of depairing, the male and female moths are to be moved side way so that the moths are separated easily without causing injury to reproductive organs.
The mated female moths are taken in a separate container and induced for urination.
Moths are placed on egg sheet and covered with cellules and kept in semi dark condition for oviposition.
Under proper preservation (5-7 0C), male moths can be used for second pairing by giving 1-2 hours rest.
Throughout the process of pairing, depairing and oviposition, optimum temperature of 25 ± 1 0C and relative humidity of 75 ± 5% should be maintained.

Dip the egg sheets in 2% formalin for 10-15 minutes. This helps in removal of pathogens adhering to the egg shell and further prevents secondary contamination.
Wash the eggs in formalin solution. It helps in firm adherence of eggs to the sheet.

Maintain temperature of 25 ± 1˚C, relative humidity of 75 ± 5%, 16 hours of light and 8 hours darkness.
During the pinhead stage or before two days of hatching, maintain total darkness for uniform development of embryo and hatching of larvae at a time on a single day.